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Ranum committed Dec 16, 2020
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# SPLiT-Seq_demultiplexing_0.1.4
This tool was created to provide an open source, portable solution for demultiplexing SPLiT-Seq RNA-Seq datasets. SPLiT-Seq_demultiplexing has two core versions:
# SPLiT-Seq_demultiplexing_0.2.1
This tool was created to provide an open source, portable solution for demultiplexing SPLiT-Seq RNA-Seq datasets. SPLiT-Seq_demultiplexing has three core versions:
1. `--version merged` which produces one .fastq file in which CellID and UMI information is appended to the readID.
2. `--version split` which produces one .fastq file for each single-cell identified. Output .fastq files are named using the identified barcode combination and UMIs are appended to the readID.
3. `--version fast` which produces one .fastq file in which CellID and UMI information is appended to the readID. The `fast` version utilizes position based barcode extraction and multithreading to deliver massively faster results compared to the original `split` and `merged` configurations.
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